Molecular Cytogenetics and Genomic Analysis in Parodontidae
The Molecular Cytogenetics and Genomic Analysis in Parodontidae has focus on the karyotypic diversification, studies of genes involved in gonadal development, molecular exaptation and the investigation of restricted DNA sequences presented on W chromosome. Parodontidae is a group of Neotropical fish with 54 conserved chromosomes with some species without heteromorphic sex chromosome systems, species with proto-sex chromosomes and species with ZZ/ZW or ZZ/ZW1W2 sex chromosome systems. Furthermore, a high amount of the karyotypic diversification observed in Parodontidae species is due to the repetitive DNA sequences movement, providing karyotypic rearrangements. Repetitive DNA sequences were isolated from W chromosome and then characterized. Using the next generation sequencing method, we compared gonadal development genes presented on ‘W chromosome’ of species bearing ZW sex chromosomes. This allowed us to evaluate the molecular co-option of transposable elements in some important regulatory regions presented on W chromosome. So, the main goal of this project is to evaluate the chromosomal diversification in fishes, investigate possible gene sequences presented on W sex chromosome and, study the molecular co-option of transposable elements involved in gene expression modulation.